High-performance thin-layer chromatography (HPTLC) is a powerful analytical technique that relies on polarity differences to separate the components of a mixture. HPTLC is used to assess substance purity and identify constituents and active substances in a mixture. Pharmaceutical analysis, food and drug analysis, environmental analysis, biochemistry, and most chem-based fields depend on HPTLC. HPTLC is an enhancement of ordinary thin-layer chromatography (TLC) and follows a nearly identical process to TLC. It will be easier to understand HPTLC if you first have a basic understanding of ordinary TLC. What is TLC? TLC is a sub-class of planar chromatography. It is...
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